Parameters related to post-GIF K3 summit camera

  • For data collected after March 2020.

Dimension: 5760 x 4092 physical pixels; 11520 x 8184 super-resolution pixels

Physical pixel side length: 5μm

Mag table (physical pixel sizes):

  • 53000x 1.638A
  • 64000x 1.346A
  • 81000x  1.068A
  • 105kx  0.832A
  • 130kx  0.651A
  • 165kx  0.511A

Magnification distortion, without rotation and flipping (estimated in April 2020 using method mag_distortion_estimate, super-resolution pixel sizes are used below):

130kx

 The following distortion parameters were found:
  • Distortion Angle = 151.5
  • Major Scale = 1.004
  • Minor Scale  = 0.996

Stretch only parameters would be as follows:

  • Distortion Angle = 151.4
  • Major Scale = 1.008
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.324
  • The Total Distortion = .80%

105kx

 The following distortion parameters were found:
  • Distortion Angle  = 153.4
  • Major Scale  = 1.006
  • Minor Scale = 0.994

Stretch only parameters would be as follows:

  • Distortion Angle = 153.4
  • Major Scale = 1.012
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.414
  • The Total Distortion = 1.20%

81kx

 The following distortion parameters were found:
  • Distortion Angle = 125.3
  • Major Scale = 1.004
  • Minor Scale  = 0.996

Stretch only parameters would be as follows:

  • Distortion Angle = 125.3
  • Major Scale = 1.000
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.532
  • The Total Distortion = .80%

64kx

 The following distortion parameters were found:
  • Distortion Angle = 143.3
  • Major Scale  = 1.004
  • Minor Scale = 0.996

Stretch only parameters would be as follows :

  • Distortion Angle = 143.3
  • Major Scale = 1.008
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.670
  • The Total Distortion = .80%

MTF data is available from Gatan: https://www.gatan.com/techniques/cryo-em#MTF