• wang-phage
    • FJS_1.1
    • FJS_2.1
    • Malvankar_Geobacter
    • Appl Environ Microbiol . 2020 87(4):e02519-20.
    • Nature . 2021 May 26. doi: 10.1038
    • yunjie-fib
    • Micrograph slide of protein
    • Micrograph slide of protein

welcome

IMPORTANT: New instructions for in-person training during COVID. 


Effective July 1, 2020, the CryoEM resources at Yale will be combined into a single functional unit as the Yale CryoEM Resource. This includes instruments currently housed across all three campuses including the Titan Krios on West Campus, the Tecnai T12 and Glacios at YSM and the Talos and Glacios instruments on Science Hill. Over the next few months, this website will be updated to reflect these changes as we transition to the new operational model. In the meantime, more detailed technical information can be found on the legacy websites below and questions can be directed to Dr. Xinran Liu, Interim Director of the Yale CryoEM Resource.
 
Yale CryoEM Resource facilities:
 
Science Hill location (formerly Science Hill CryoEM facility): Thermo Scientific Talos L120C at Bass Center, Rm 231 (266 Whitney Ave), Thermo Scientific Glacios at Yale Science Building, Rm C133 (260 Whitney Ave)
YSM location (formerly part of CCMI): FEI Tecnai T12 and Thermo Scientific Glacios at Sterling Hall of Medicine IE 16/26 (333 Cedar Street)
West Campus Location: FEI Titan Krios G2 at West Campus Advanced Biosciences Center
Both new and existing users of these resources will need to set up an account in the FOM system to apply for training and make reservations on equipment in the consolidated facility:
 
Get an active Workday COA (Charter of Account) number from your advisor.
Check to make sure your computer/smartphone is on Yale local network or Yale VPN.
Click Yale FOM (Facility Online Management) to open Yale FOM page and login using your NetID and password.
Follow instructions to set up your personal FOM account. Let us know if your PI is not in our supervisor list.
Follow the requested format to fill COA number in the account setup page.
Fill and submit the biosafety form to activate “Add this account” button.
Go to “User Home” page, click the small square button next to “Yale CryoEM Facility” and select the instrument which you would like to access.
In the popup “New User Application Form”  choose “Internal” user and hit “Apply” button. The Core manager will be informed by email and you will be contacted for training arrangements.

Announcements

  • maintenance

    West campus Cryo-EM Core status

    Current status:   8/16/2021 to present - Major stage/holder service. 8/16 - pre-service test, warm up system. 8/17 - ppal vacuum erroneously reported log1, to be replaced, order pirani penning gauge kf25. chiller off, lenses off, detectors warmed up, GIF/K3 power off, phase plate heater off, Igpf off, column split, remove old stage and holder. 8/18 - install new stage and holder. Chiller back on. Install software (not finished). 8/19 -  use the old DT holder for enabling (workaround), remove ST holder to insert a XG grid, re-install ST holder. 8/20 - order winch and band. 8/23 - replace hoist winch and band (belt worn out, slipping), borrow a clevis from NYC, order a new clevis kit, rebuilt column, pump vacuum. 8/24 - turn on beam, tune stage position, alpha tilt. 8/25 - chiller down, recover chiller, reset optics power, tune stage position. Support PC windows 7 not genuine message. 8/26 - test eucentricity, defocus. Clone hard drive. 8/27 - install software (not finished). 8/30 - troubleshoot software. 8/31 - warm up column. 9/1 - split column, pump vacuum. 9/2 - tune rough exchange position, rebult column, pump vacuum. 9/3 - C1 aperture error (Y sticky), manually center the C1 aperture, disconnect microE, LAN cable, to be replaced at tip change.  9/7 - the EPD bracket and lead cover from the new stage didn’t fit, swap to the bracket and lead cover from the old stage.  backup microscope settings. 9/8 - HT on, align optics, GIB, K3 power on. 9/9 - reinstall OS and software on the microscope PC from scratch. TUI 2.15.4. 9/10 - update storage server, install patches, clone harddrive. 9/13 - EPU/FEI tomography have license issues. 9/14 - fluscreen battery needs to be replaced (voltage very low at ~5V, should be 22.5V), order from Amazon. 9/15 - load standard specimens, test resolution. 9/16 - align phase plate, redo fluscreen gain. Flu cam crash (memory?) caused Tem Server crash (BrickBox error). Recover Tem server, HT. 9/17 - replace ppal, chilled water filter. hoist band still slips. 9/20,21 - align optics. 9/22 - stage calibration. Scheduled maintenance: None Planned maintenance: Major stage/holder service.  Annual PM. Gatan update. Other works that may interrupt the operation in the future: None. Past services
  • Yale FOM Registration

    June 22, 2020
    Yale researchers please follow the steps below for Yale FOM account setup and instrument application. For Krios, the staff will book the instrument on behalf of the user, but the user must be registered in the system. A specific combination of one or multiple COA accounts (aka. charging instructions) is linked to a specific project. If the user chooses to switch COAs, he must first add those new COAs to his account and project in FOM system. In other words, the user needs to provide the charging instruction twice in the process, first is in the Access request form, second is in the FOM system. The latter may be skipped if the project and its corresponding charging instruction already exist. This will allow the staff to select the appropriate charging instructions for the corresponding project in FOM when preparing billing reports.  This instruction is adapted from https://ywcmatsci.yale.edu/registration Get an active Workday COA (Charter of Accounts) number from your advisor. Check to make sure your computer/smartphone is on Yale local network or Yale VPN. Click Yale FOM (Facility Online Management) to open Yale FOM page and logon using your NetID and password. Follow instructions to set up your personal FOM account. Let us know if your PI is not in our supervisor list. Follow the requested format to fill COA number in the accout setup page. Fill and submit the biosafety form to activate “Add this account” button. Existing FOM users can also click “My Accounts” in your FOM account to add a new account. An updated biosafety form is required for each new COA account. Go to “User Home” page, click the small square button next to “Yale cryo-em facility” below “Available Equipment” to open the dropdown instrument. Find the WC Titan Krios and activate it. It should show up in the Authorized equipments on the left side.