• Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Example Micrograph
    • Micrograph slide of protein

welcome

Yale University’s Electron Cryo-Microscopy resources encompass a broad range of Cryo-EM imaging instrumentation dedicated to bringing state of the art technology to bear on the most provocative questions in structural biology research.

Our resources include:

The Titan Krios is the world’s most powerful microscope for three-dimensional characterization of biological molecules. It is optimized for life science applications, enabling the visualization of molecules in atomic detail via both single-particle Cryo-EM and Electron Cryo Tomography.

Our facilities are open to new and experienced users. Please see specifics of each instrument for training and schedule information.

Announcements

maintenance
Current status:   04/17/2019 - K2 failure, ADC sync error, wait for service. 04/22/2019 - K2 remote service by Gatan. ADC board needs replacement. 05/08/2019 - HP Apollo movie frame storage server failure; Stage tracking error, No measurement Axis Y. Wait for Thermo service. 5/9/2019 - Gatan field service, replace ADC assembly in the digitizer, ADC sync error remains. 5/10/2019 - Remove K2 digitizer from the microscope, ship it back to the factory. Detector unit to be uninstalled and shipped.  5/13/2019 to 5/17/2019 - Thermo service. Stage encoder (Y MicroE) replaced and is back to operational on 5/16.  HP Apollo movie frame storage server’s battery needs replacement, wait for delivery from HP. 5/23/2019 - Replace HP Apollo battery by Thermo. Uninstall K2 by Gatan, ship back to the factory. 6/4/2019 - Repair of K2 continues at the factory. Parts to be replaced: Thermal pads, Cylinder Block, Plugs, Fitting, O-Rings, Drive rods, Pistons, Washers, Assembly Sensor. Scheduled maintenance: None. Planned maintenance: Reinstall K2. Other works that may interrupt the operation in the future: Lay network cables from 059F to 059A and move Falcon data server to 059A. Past services
FEG
Jan.02.2017 – Crates arrived Jan. 05. 2017 – Unpacking and installation started Jan.12.2017 – Microscope body rolled into enclosure Feb.10.2017 – The gun beamed for the first time on site Feb.28.2017 – Installation of GIF/K2 started Mar.29.2017 – Sign-off July.01.2017 – Open to Yale community ♦ Photo credit: Chris Incarvito
Introduction (Michael C. Crair) Overview of Cryo-EM at Yale (Xinran Liu) (Yong Xiong) Introduction to Titan Krios (Shenping Wu) Computational magic behind single-particle Cryo-EM (Fredrick J. Sigworth) From X-Ray crystallography to Cryo-EM: Single-particle applications (Yong Xiong) The future of cryo-electron tomography: Cell biology at high resolution (Jun Liu)