• Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Micrograph slide of protein
    • Example Micrograph
    • Micrograph slide of protein

welcome

Effective July 1, 2020, the CryoEM resources at Yale will be combined into a single functional unit as the Yale CryoEM Resource. This includes instruments currently housed across all three campuses including the Titan Krios on West Campus, the Tecnai T12 and Glacios at YSM and the Talos and Glacios instruments on Science Hill. Over the next few months, this website will be updated to reflect these changes as we transition to the new operational model. In the meantime, more detailed technical information can be found on the legacy websites below and questions can be directed to Dr. Xinran Liu, Interim Director of the Yale CryoEM Resource.
 
Yale CryoEM Resource facilities:
 
Science Hill location (formerly Science Hill CryoEM facility): Thermo Scientific Talos L120C at Bass Center, Rm 231 (266 Whitney Ave), Thermo Scientific Glacios at Yale Science Building, Rm C133 (260 Whitney Ave)
YSM location (formerly part of CCMI): FEI Tecnai T12 and Thermo Scientific Glacios at Sterling Hall of Medicine IE 16/26 (333 Cedar Street)
West Campus Location: FEI Titan Krios G2 at West Campus Advanced Biosciences Center
Both new and existing users of these resources will need to set up an account in the FOM system to apply for training and make reservations on equipment in the consolidated facility:
 
Get an active Workday COA (Charter of Account) number from your advisor.
Check to make sure your computer/smartphone is on Yale local network or Yale VPN.
Click Yale FOM (Facility Online Management) to open Yale FOM page and login using your NetID and password.
Follow instructions to set up your personal FOM account. Let us know if your PI is not in our supervisor list.
Follow the requested format to fill COA number in the account setup page.
Fill and submit the biosafety form to activate “Add this account” button.
Go to “User Home” page, click the small square button next to “Yale CryoEM Facility” and select the instrument which you would like to access.
In the popup “New User Application Form”  choose “Internal” user and hit “Apply” button. The Core manager will be informed by email and you will be contacted for training arrangements.

Announcements

maintenance
Current status:   Operational. The facility is open to qualified projects in phase 1. Scheduled maintenance: None. Planned maintenance: License update. Other works that may interrupt the operation in the future: None. Past services
The facility transitions into phase 1 on June 4. First of all,  this guide applies to all people who will be present on Yale’s campus during Phase 1. Please also follow the west campus specific updates here https://westcampus.yale.edu/yale-west-campus-covid-19-updates . Additional guidelines: 1) Users that need to work in the prep room should wear their personal lab coat. Their supervisors shall supply them with masks to be used in the facility. 2) Users that need assistance to enter the building can call the lab phone. 3) No unannounced in-person visits are allowed (feel free to call the lab phone if you want to discuss something with the staff). Anyone that plans to visit the lab, either because he has a scheduled experiment or for other purpose, needs to communicate with the staff first. A maxium of two people (one staff member and one user) are allowed in the control room.  4) All occupants need to sign in to a google calendar that is set up to keep track of visitors. The staff will do that on behalf of the users during weekdays, but users need to do it themselves on weekends/holidays. Instructions will be given to individuals upon their visits.  5) The doors to the EM suite shall be kept closed. 
Please check out opportunities of Titan Krios access at other places when the Yale Krios is undergoing upgrade and maintenance.  NIH free resources: https://cryoem.yale.edu/user-guidelines/national-cryo-em-centers HHMI eligible: Janelia. Other places that are known to accept paid external users (not a complete list):  https://cores.research.asu.edu/materials/equipment/titan-krios-fei http://cores.utah.edu/electron-microscopy/#1518120922177-6c1c910e-5b39 https://bsir.bio.fsu.edu/facility-rates https://wucci.wustl.edu/user-info/rates/ https://cryoem.hms.harvard.edu/users https://www.umassmed.edu/research/cores/cryo-em-core-facility/contact/ https://cryoem.bio.purdue.edu/services/index.html http://cryoem.jhmi.edu/scheduling/ https://iac.princeton.edu/equipment.html https://smif.pratt.duke.edu/node/55048 https://cemas.osu.edu/Rates https://case.edu/medicine/research/som-core-facilities/cryo-electron-mic… https://www.med.upenn.edu/electronmicroscopyresourcelab/request-access.html
June 22, 2020
Yale researchers please follow the steps below for Yale FOM account setup and instrument application. For Krios, the staff will book the instrument on behalf of the user, but the user must be registered in the system. A specific combination of one or multiple COA accounts (aka. charging instructions) is linked to a specific project. If the user chooses to switch COAs, he must first add those new COAs to his account and project in FOM system. In other words, the user needs to provide the charging instruction twice in the process, first is in the Access request form, second is in the FOM system. The latter may be skipped if the project and its corresponding charging instruction already exist. This will allow the staff to select the appropriate charging instructions for the corresponding project in FOM when preparing billing reports.  This instruction is adapted from https://ywcmatsci.yale.edu/registration Get an active Workday COA (Charter of Accounts) number from your advisor. Check to make sure your computer/smartphone is on Yale local network or Yale VPN. Click Yale FOM (Facility Online Management) to open Yale FOM page and logon using your NetID and password. Follow instructions to set up your personal FOM account. Let us know if your PI is not in our supervisor list. Follow the requested format to fill COA number in the accout setup page. Fill and submit the biosafety form to activate “Add this account” button. Existing FOM users can also click “My Accounts” in your FOM account to add a new account. An updated biosafety form is required for each new COA account. Go to “User Home” page, click the small square button next to “Yale cryo-em facility” below “Available Equipment” to open the dropdown instrument. Find the WC Titan Krios and activate it. It should show up in the Authorized equipments on the left side.