Instrument Parameters

Acceleration voltage: 300kV

Cs of Cryo-TWIN objective lens: 2.7mm

  • Available C2 aperture sizes (μm): 150, 100, 70, 50
  • Available objective aperture sizes (μm): 100, 70

Dimension: 4096 x 4096 pixels
Physical pixel side length: 14μm
 

Mag table (pixel sizes calibrated using reconstructions of T20S):

  • 47000x  1.706A
  • 59000x  1.36A
  • 75000x  1.07A
  • 96000x  0.831A

Magnification distortion (estimated in June 2017 using method mag_distortion_estimate)

SA 75000x

The following distortion parameters were found:

  • Distortion Angle  = 88.6
  • Major Scale = 1.002
  • Minor Scale = 0.998

Stretch only parameters would be as follows:

  • Distortion Angle = 88.6
  • Major Scale = 1.004
  • Minor Scale = 1.000
  • Corrected Pixel Size = 1.068
  • The Total Distortion = .35%

  • For data collected before March 2020.
     

Dimension: 3838 x 3710 physical pixels; 7676 x 7420 super-resolution pixels
Physical pixel side length: 5μm

Mag table (physical pixel sizes):

  • 81000x  1.715A
  • 105kx  1.333A
  • 130kx  1.05A
  • 165kx  0.822A

Magnification distortion (estimated in June 2017 using method mag_distortion_estimate, super-resolution pixel sizes are used below):

165kx

The following distortion parameters were found:

  • Distortion Angle = 147.3
  • Major Scale = 1.005
  • Minor Scale  = 0.995

Stretch only parameters would be as follows:

  • Distortion Angle = 147.3
  • Major Scale = 1.010
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.409
  • The Total Distortion = 1.00%

130kx

The following distortion parameters were found:

  • Distortion Angle  = 141.1
  • Major Scale  = 1.005
  • Minor Scale = 0.996

Stretch only parameters would be as follows:

  • Distortion Angle = 141.1
  • Major Scale = 1.009
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.523
  • The Total Distortion = .90%

105kx

The following distortion parameters were found:

  • Distortion Angle = 143.0
  • Major Scale = 1.007
  • Minor Scale  = 0.994

Stretch only parameters would be as follows:

  • Distortion Angle = 143.0
  • Major Scale = 1.013
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.662
  • The Total Distortion = 1.30%

81kx

The following distortion parameters were found:

  • Distortion Angle = 149.5
  • Major Scale  = 1.004
  • Minor Scale = 0.996

Stretch only parameters would be as follows :

  • Distortion Angle = 149.5
  • Major Scale = 1.008
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.854
  • The Total Distortion = .75%

MTF star file (estimated in June 2017 using method FindDQE)

  • For data collected after March 2020.
     

Dimension: 5760 x 4092 physical pixels; 11520 x 8184 super-resolution pixels

Physical pixel side length: 5μm

Mag table (physical pixel sizes):

  • 53000x 1.638A
  • 64000x 1.346A
  • 81000x  1.068A
  • 105kx  0.832A
  • 130kx  0.651A
  • 165kx  0.511A

Magnification distortion, without rotation and flipping (estimated in April 2020 using method mag_distortion_estimate, super-resolution pixel sizes are used below):

130kx

The following distortion parameters were found:

  • Distortion Angle = 151.5
  • Major Scale = 1.004
  • Minor Scale  = 0.996

Stretch only parameters would be as follows:

  • Distortion Angle = 151.4
  • Major Scale = 1.008
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.324
  • The Total Distortion = .80%

105kx

The following distortion parameters were found:

  • Distortion Angle  = 153.4
  • Major Scale  = 1.006
  • Minor Scale = 0.994

Stretch only parameters would be as follows:

  • Distortion Angle = 153.4
  • Major Scale = 1.012
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.414
  • The Total Distortion = 1.20%

81kx

The following distortion parameters were found:

  • Distortion Angle = 125.3
  • Major Scale = 1.004
  • Minor Scale  = 0.996

Stretch only parameters would be as follows:

  • Distortion Angle = 125.3
  • Major Scale = 1.000
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.532
  • The Total Distortion = .80%

64kx

The following distortion parameters were found:

  • Distortion Angle = 143.3
  • Major Scale  = 1.004
  • Minor Scale = 0.996

Stretch only parameters would be as follows :

  • Distortion Angle = 143.3
  • Major Scale = 1.008
  • Minor Scale = 1.000
  • Corrected Pixel Size = 0.670
  • The Total Distortion = .80%

MTF data is available from Gatan: https://www.gatan.com/techniques/cryo-em#MTF