Publications

Publications Supported by Yale CryoEM Resource

Saccharibacteria deploy two distinct type IV pili, driving episymbiosis, host competition, and twitching motility ISME J
The Flagellin-Specific Chaperone FliS of Borrelia burgdorferi Controls the Cytoplasmic Pool of Flagellins at the Level of Translation Initiation, Secretion, and Proteolysis Mol Microbiol
Outer membrane tube formation by Francisella novicida involves extensive envelope modifications and is linked with type VI secretion and alterations to the host phagosomal membrane mBio
The mechanochemical cycle of reactive full-length human dynein 1 Nat Struct Mol Biol
Cryo-EM structure of the brine shrimp mitochondrial ATP synthase suggests an inactivation mechanism for the ATP synthase leak channel Cell Death Differ
Borrelia burgdorferi serine protease HtrA is a pleiotropic regulator of stress response, motility, flagellar hemostasis, and infectivity Commun Biol
CryoEM structures of Kv1.2 potassium channels, conducting and non-conducting eLife
Tetrameric PilZ protein stabilizes stator ring in complex flagellar motor and is required for motility in Campylobacter jejuni PNAS
FlbB forms a distinctive ring essential for periplasmic flagellar assembly and motility in Borrelia burgdorferi PLoS Pathog
Structure and evolution of photosystem I in the early-branching cyanobacterium Anthocerotibacter panamensis PNAS
High-resolution structures of Myosin-IC reveal a unique actin-binding orientation, ADP release pathway, and power stroke trajectory PNAS
Structure of a mutated photosystem II complex reveals changes to the hydrogen-bonding network that affect proton egress during O-O bond formation J Biol Chem
Structural insights into HIV-2 CA lattice formation and FG-pocket binding revealed by single-particle cryo-EM Cell Rep
Molecular insights into de novo small-molecule recognition by an intron RNA structure PNAS
FlgY, PflA, and PflB form a spoke–ring network in the high-torque flagellar motor of Helicobacter pylori PNAS
Structural basis for the interaction between the Drosophila RTK Sevenless (dROS1) and the GPCR BOSS Nat Commun
A Helicobacter pylori flagellar motor accessory is needed to maintain the barrier function of the outer membrane during flagellar rotation PLoS Pathog

Broadly conserved FlgV controls flagellar assembly and Borrelia burgdorferi dissemination in mice Nat Commun
Multiple mechanisms for licensing human replication origins Nature
Cryo-EM structure of a photosystem I variant containing an unusual plastoquinone derivative in its electron transfer chain Sci Adv
PilY1 regulates the dynamic architecture of the type IV pilus machine in Pseudomonas aeruginosa Nat Commun
Elucidating bacterial coaggregation through a physicochemical and imaging surface characterization Sci Total Environ
High-resolution in situ structures of mammalian respiratory supercomplexes Nature
Dual function of LapB (YciM) in regulating Escherichia coli lipopolysaccharide synthesis PNAS
Outer membrane vesicles and the outer membrane protein OmpU govern Vibrio cholerae biofilm matrix assembly mBio
Episymbiotic Saccharibacteria TM7x modulates the susceptibility of its host bacteria to phage infection and promotes their coexistence PNAS
Structure of the antenna complex expressed during far-red light photoacclimation in Synechococcus sp. PCC 7335 J Biol Chem
Structure of a biohybrid photosystem I-platinum nanoparticle solar fuel catalyst Nat Commun
Structure and inhibition of SARS-CoV-2 spike refolding in membranes Science
Mutation-induced shift of the photosystem II active site reveals insight into conserved water channels J Biol Chem
Structure of mavacamten-free human cardiac thick filaments within the sarcomere by cryoelectron tomography PNAS
The molecular basis of sugar detection by an insect taste receptor Nature
Native architecture of a human GBP1 defense complex for cell-autonomous immunity to infection Science
GABAA receptor π forms channels that stimulate ERK through a G-protein-dependent pathway Mol Cell
Mechanistic basis for the translation inhibition of Cutibacterium acnes by Clindamycin J Invest Dermatol
A pleiotropic role of sialidase in the pathogenicity of Porphyromonas gingivalis Infect Immun
Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 complex Nat Commun
Bacterial flagella hijack type IV pili proteins to control motility PNAS

Structural insights into intron catalysis and dynamics during splicing Nature
Abl2 repairs microtubules and phase separates with tubulin to promote microtubule nucleation Curr Biol
Cryo-EM reveals how the mastigoneme assembles and responds to environmental signal changes J Cell Biol
Microtubule-binding-induced allostery triggers LIS1 dissociation from dynein prior to cargo transport Nat Struct Mol Biol
Anti-s28 Factor FlgM Regulates Flagellin Gene Expression and Flagellar Polarity of Treponema denticola J Bacteriol
Structure of a dimeric photosystem II complex from a cyanobacterium acclimated to far-red light J Biol Chem
A dual role for the chromatin reader ORCA/LRWD1 in targeting the origin recognition complex to chromatin EMBO J 
A protocol for capturing RNA-sensing innate immune receptors in multiple conformations by single-particle cryo-EM STAR Protoc
Helical allophycocyanin nanotubes absorb far-red light in a thermophilic cyanobacterium Sce Adv
Cryo-EM analyses of KIT and oncogenic mutants reveal structural oncogenic plasticity and a target for therapeutic intervention PNAS
LRRC23 truncation impairs radial spoke 3 head assembly and sperm motility underlying male infertility eLife
HIV-1 Env trimers asymmetrically engage CD4 receptors in membranes Nature
Structural basis for translation inhibition by MERSCoV Nsp1 reveals a conserved mechanism for betacoronaviruses Cell Report
Sarecycline inhibits protein translation in Cutibacterium acnes 70S ribosome using a two-site mechanism Nucleic Acids Res
Structural basis for recruitment of host CypA and E3 ubiquitin ligase by maedi-visna virus Vif Sci Adv 
FlgV forms a flagellar motor ring that is required for optimal motility of Helicobacter pylori PLoS One
Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 °C eLife
Diacylglycerol-dependent hexamers of the SNARE-assembling chaperone Munc13-1 cooperatively bind vesicles PNAS
The E3 ligase Riplet promotes RIG-I signaling independent of RIG-I oligomerization Nat Commun
Parkinson’s disease kinase LRRK2 coordinates a cell-intrinsic itaconate-dependent defence pathway against intracellular Salmonella Nat Microbiol
Gut colonization by Bacteroides requires translation by an EF-G paralog lacking GTPase activity EMBO J 
Bacteria require phase separation for fitness in the mammalian gut Science
High-Resolution Structural Analysis of Dyneins by Cryo-electron Microscopy Methods Mol Biol

Structures of a mobile intron retroelement poised to attack its structured DNA target Science
Transcriptome of Epibiont Saccharibacteria Nanosynbacter lyticus Strain TM7x During the Establishment of Symbiosis J Bacteriol
Exploitation of a Bacterium-Encoded Lytic Transglycosylase by a Human Oral Lytic Phage To Facilitate Infection J Virol
Cryo-EM structures of prefusion SIV envelope trimer. Nat Struct Mol Biol
Regulatory mechanisms of lipopolysaccharide synthesis in Escherichia coli Nat Commun
Identification and Characterization of the Alternative σ28 Factor in Treponema denticola J Bacteriol
Multi-curve fitting and tubulin-lattice signal removal for structure determination of large microtubule-based motors J Struct Biol
Monobody adapter for functional antibody display on nanoparticles for adaptable targeted delivery applications Nat Commun
The flagellar motor protein FliL forms a scaffold of circumferentially positioned rings required for stator activation PNAS
FliL ring enhances the function of periplasmic flagella PNAS
Cryo-EM structure of an active central apparatus Nat Struct Mol Biol
Transcriptional and functional characterizations of multiple flagellin genes in spirochetes Mol Microbiol
Structural insights into the mechanism of the sodium/iodide symporter  (NIS) Nature
The RIG-I receptor adopts two different conformations for distinguishing host from viral RNA ligands Mol Cell
Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction PNAS
Munc13 structural transitions and oligomers that may choreograph successive stages in vesicle priming for neurotransmitter release PNAS
High-resolution cryo-electron microscopy structure of photosystem II from the mesophilic cyanobacterium, Synechocystis sp. PCC 6803 PNAS
Single particle cryo-EM structure of the outer hair cell motor protein prestin Nat Commun
Structures of the CcmABCD heme release complex at multiple states Nat Commun
How to correct relative voxel scale factors for calculations of vector-difference Fourier maps in cryo-EM J Struct Biol
In situ architecture of the lipid transport protein VPS13C at ER–lysosome membrane contacts PNAS
Developmental transitions coordinate assembly of the Coxiella burnetii Dot/Icm type IV secretion system Infect Immun
Structural basis and mode of action for two broadly neutralizing antibodies against SARS-CoV-2 emerging variants of concern Cell Rep
Monospecific and bispecific monoclonal SARS-CoV-2 neutralizing antibodies that maintain potency against B.1.617 Nat Commun 
An essential periplasmic protein coordinates lipid trafficking and is required for asymmetric polar growth in mycobacteria eLife
A Tripartite Efflux System Affects Flagellum Stability in Helicobacter pylori Int J Mol Sci

BB0259 Encompasses a Peptidoglycan Lytic Enzyme Function for Proper Assembly of Periplasmic Flagella in Borrelia burgdorferi Front Microbiol
Characterization of the Flagellar Collar Reveals Structural Plasticity Essential for Spirochete Motility mBio
Structure and analysis of nanobody binding to the human ASIC1a ion channel eLife
Cryo-EM structure of the needle filament tip complex of the Salmonella type III secretion injectisome PNAS
Structure of Geobacter pili reveals secretory rather than nanowire behaviour Nature
Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism Nat Struct Mol Biol 
Structural basis of mismatch recognition by a SARS-CoV-2 proofreading enzyme Science
Cryo-EM structures of Escherichia coli cytochrome bo3 reveal bound phospholipids and ubiquinone-8 in a dynamic substrate binding site PNAS
Nodal modulator (NOMO) is required to sustain endoplasmic reticulum morphology J Biol Chem 
A human apolipoprotein L with detergent-like activity kills intracellular pathogens Science
A mammalian system for high-resolution imaging of intact cells by cryo-electron tomography Prog Biophys Mol Biol
Symmetrical arrangement of proteins under release-ready vesicles in presynaptic terminals PNAS
A phase-separated nuclear GBPL circuit controls immunity in plants Nature
Structural visualization of transcription activated by a multidrug-sensing MerR family regulator Nat Commun 
Interconnecting solvent quality, transcription, and chromosome folding in Escherichia coli Cell
Role of the major determinant of polar flagellation FlhG in the endoflagella- containing  spirochete Leptospira Mol Microbiol
A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi Appl Environ Microbiol
Composition and Biophysical Properties of the Sorting Platform Pods in the Shigella Type III Secretion System Front Cell Infect Microbiol

Cryo-EM Structure of Monomeric Photosystem II from Synechocystis sp. PCC 6803 Lacking the Water-Oxidation Complex Joule
Dynamic and asymmetric fluctuations in the microtubule wall captured by high-resolution cryoelectron microscopy PNAS
Structures of cofilin-induced structural changes reveal local and asymmetric perturbations of actin filaments PNAS
The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching eLife
Nonstructural Protein 1 of SARS-CoV-2 Is a Potent Pathogenicity Factor Redirecting Host Protein Synthesis Machinery toward Viral RNA Mol Cell
Cryo-electron microscopy structures of pyrene-labeled ADP-Pi- and ADP-actin filaments Nat Commun 
Structural basis for the activation and suppression of transposition during evolution of the RAG recombinase EMBO J 
Molecular dysregulation of ciliary polycystin-2 channels caused by variants in the TOP domain PNAS
Structure of the human cation-chloride cotransporter NKCC1 determined by single-particle electron cryo-microscopy Nat Commun
Molecular mechanism for rotational switching of the bacterial flagellar motor Nat Struct Mol Biol
Sub-Nanometer Structures of HIV-1 Envelope Trimers on Aldrithiol-2 Inactivated Virus Particles Nat Struct Mol Biol
Insights into Lysosomal PI(3,5)P2 Homeostasis from a Structural-Biochemical Analysis of the PIKfyve Lipid Kinase Complex Mol Cell
Electric field stimulates production of highly conductive microbial OmcZ nanowires Nat Chem Biol
c-di-GMP modulates type IV MSHA pilus retraction and surface attachment in Vibrio cholerae Nat Commun 
Cryo-EM reconstruction of a VPS13 fragment reveals a long groove to channel lipids between membranes J Cell Biol
FlhF regulates the number and configuration of periplasmic flagella in Borrelia burgdorferi Mol Microbiol
BB0326 is responsible for the formation of periplasmic flagellar collar and assembly of the stator complex in Borrelia burgdorferi Mol Microbiol

Structural basis of antagonism of human APOBEC3F by HIV-1 Vif Nat Struct Mol Biol
A mitochondrial megachannel resides in monomeric F1FO ATP synthase Nat Commun
High-resolution view of the type III secretion export apparatus in situ reveals membrane remodeling and a secretion pathway PNAS
Structures of a RAG-like transposase during cut-and-paste transposition Nature
A polymorphic helix of a Salmonella needle protein relays signals defining distinct steps in type III secretion PLoS Biol
Structural basis for the clamping and Ca2+ activation of SNARE-mediated fusion by synaptotagmin Nat Commun
Structure of Microbial Nanowires Reveals Stacked Hemes that Transport Electrons over Micrometers Cell
Mechanism of actin polymerization revealed by cryo-EM structures of actin filaments with three different bound nucleotides PNAS
Symmetrical organization of proteins under docked synaptic vesicles FEBS Lett
Structural dynamics of bacteriophage P22 infection initiation revealed by cryo-electron tomography Nat Microbiol

The actin filament twist changes abruptly at boundaries between bare and cofilin-decorated segments J Biol Chem
Structural basis of the filamin A actin-binding domain interaction with F-actin Nat Struct Mol Biol
Visualization of the type III secretion mediated Salmonella–host cell interface using cryo-electron tomography eLife
FcpB Is a Surface Filament Protein of the Endoflagellum Required for the Motility of the Spirochete Leptospira Front Cell Infect Microbiol