Yale TEM and support equipment

300-kV Titan Krios G2 Transmission Electron Microscope 

• High-brightness X-FEG gun
• ConstantPower lenses
• Three condenser lens system for parallel sample illumination
• Robotic Autoloader handles and stores up to 12 EM grids
• Volta phase plate
• GIF Quantum LS Imaging Filter Model 1967
• Pre-GIF CETA2 indirect detection CMOS camera
• Pre-GIF Falcon 3EC direct detection camera
• Post-GIF K3 summit direct detection camera
• EPU: Automated Data Acquisition Software for Single Particle Analysis
• TEM Tomography Data Acquisition Software
• Gatan Microscopy Suite® (GMS) 3.43
• Microscope PC OS: Win7
• SerialEM (for K3)

View the Installation Timeline
 

Instruments for sample preparation
 

• PELCO easiGlow™ Glow Discharge Cleaning System
  Optimized for cleaning TEM grids
• FEI Vitrobot™ Mark IV

200-kV YSM-Glacios:

• X-FEG gun
• ConstantPower Objective lens
• Autoloader handles and stores up to 12 EM grids
• Cryo-stage with single axis holder
• Volta phase plate
• CETA CMOS camera
• K3 direct detection camera
• EPU Software (for CETA)
• SerialEM (for K3)

120-kV YSM-Tecnai T12:

• LaB6 cathode
• Cryo-stage with side entry holder
• Gatan CCD camera
• SerialEM

Instruments for sample preparation

             o SPT Labtech Chameleon

             o FEI Vitrobot™ Mark IV

200-kV SH-Glacios:

• High-brightness X-FEG gun
• ConstantPower Objective lens
• Robotic Autoloader handles and stores up to 12 EM grids
• Cryo-stage with single axis holder
• CETA-D CMOS camera
• K3 summit direct detection camera
• EPU-D Software (for MircoED)
• SerialEM (for K3)

120-kV SH-Talos L120C:

• LaB6 cathode
• ConstantPower Objective lens
• Cryo-stage with side entry holder
• Motorized Apertures
• Digital FluCam
• CETA CMOS camera
• SerialEM

Here is an incomplete list of EM related instruments at other Yale facilities or labs.

• Talos L120c at Department of Neuroscience (co-managed by CCMI)

• T12, TF20, Zeiss dual beam, and Aquilos Cryo-FIB at CCMI

Tecnai T12 BioTwin (kept at 80kV), Tecnai F20 X-Twin, Zeiss Crossbeam 550 FIB-SEM, Aquilos 2

• FIB-SEM Collaboration core at YSM

Zeiss eFIB-SEM

• JEOL TEM at West campus

JEOL JEM 1400-plus 120 kV (kept at 80kV); LaB6 filament; bottom-mount AMT XR611 camera. Located at ISTC 006F.  Potential users should contact Chenxiang Lin (chenxiang.lin@yale.edu (link sends e-mail)).

• CryoFIB at West campus

Thermo Scientific Aquilos at Jun Liu lab

Addtional equipment includes Leica GP2 Plunge Freezer and  Leica Cryo-CLEM.

• Yale Institute for Nanoscience & Quantum Engineering

FEI Tecnai Osiris 200kV TEM

• ACEM core at West campus

Thermo Scientific Helios FIB SEM Dual Beam

Thermo Scientific Spectra Ultra STEM