Yale TEM and Support Equipment

WORK

The Yale CryoEM Resource (YCR) is a state-of-the-art facility dedicated to cryo-electron microscopy. It currently houses multiple cryo-sample preparation devices (such as the Vitrobot and Chameleon) and five electron microscopes distributed across three Yale campuses: a Titan Krios on West Campus; a Glacios and a Tecnai T12 at the Yale School of Medicine site (formerly part of CCMI); and a Glacios and a Talos L120C on Science Hill.

Details of instruments at each site are provided below

300-kV Titan Krios G2 Transmission Electron Microscope 

  • X-FEG gun
  • ConstantPower lenses
  • Three condenser lens system for parallel sample illumination
  • Autoloader handles and stores up to 12 EM grids
  • Volta phase plate
  • GIF Quantum LS Imaging Filter Model 1967
  • Pre-GIF CETA2 indirect detection CMOS camera
  • Pre-GIF Falcon 3EC direct detection camera
  • Post-GIF K3 summit direct detection camera
  • EPU: Automated Data Acquisition Software for Single Particle Analysis
  • TEM Tomography Data Acquisition Software
  • Gatan Microscopy Suite® (GMS) 3.43
  • SerialEM (for K3)

Instruments for sample preparation

              o PELCO easiGlow™ Glow Discharge Cleaning System
              o FEI Vitrobot™ Mark IV

200-kV YSM-Glacios:

  • X-FEG gun
  • ConstantPower Objective lens
  • Autoloader handles and stores up to 12 EM grids
  • Cryo-stage with single axis holder
  • Volta phase plate
  • CETA CMOS camera
  • K3 direct detection camera
  • EPU Software (for CETA)
  • SerialEM (for K3)

120-kV YSM-Tecnai T12:

  • LaB6 cathode
  • Cryo-stage with side entry holder
  • Gatan CCD camera
  • SerialEM

Instruments for sample preparation

             o SPT Labtech Chameleon

             o FEI Vitrobot™ Mark IV

             o Leica EM ACE600 High-Vacuum Coater

             o Gatan Solarus Advanced Plasma System (Model 950)

 

200-kV SH-Glacios:

  • X-FEG gun
  • ConstantPower Objective lens
  • Autoloader handles and stores up to 12 EM grids
  • Cryo-stage with single axis holder
  • CETA-D CMOS camera
  • K3 summit direct detection camera
  • EPU-D Software (for MircoED)
  • SerialEM (for K3)

120-kV SH-Talos L120C:

  • LaB6 cathode
  • ConstantPower Objective lens
  • Cryo-stage with side entry holder
  • Motorized Apertures
  • Digital FluCam
  • CETA CMOS camera
  • SerialEM

Instruments for sample preparation

              o PELCO easiGlow™ Glow Discharge Cleaning System

              o FEI Vitrobot™ Mark IV

              o Fischione: Model 1070 NanoClean

              o UVOCS: UV Ozone Cleaning System (model T10X10/OES)

              o Cressington: Carbon Coater 208 

Here is an incomplete list of EM related instruments at other Yale facilities or labs.

  • Aquilos CryoFIB, Leica GP2 Plunge Freezer, and  Leica Cryo-CLEM at Jun Liu lab
  • Aquilos CryoFIB, Zeiss dual beam, T12, and TF20 at CCMI
  • Talos L120c at Department of Neuroscience (co-managed by CCMI)
  • Zeiss eFIB-SEM at FIB-SEM Collaboration core
  • JEOL JEM 1400-plus at Chenxiang Lin Lab
  • FEI Tecnai Osiris 200kV TEM YINQE
  • Thermo Scientific Spectra Ultra STEM and Helios G4 UX DualBeam System at ACEM core