Training Opportunities at YCR

The Yale CryoEM Resource (YCR) offers a range of training opportunities to support users at different levels of experience. These include monthly virtual workshops and in-person training sessions, both designed to enhance user skills and support high-quality CryoEM research.

Virtual Workshops

YCR hosts free, one-day virtual workshops via Zoom on the last Wednesday of each month, alternating between basic and advanced sessions. These workshops do not include hands-on practice, due to time constraints, but are intended to:

  • Introduce or reinforce key CryoEM concepts
  • Address topics users may have missed during instrument training
  • Provide workflow updates and best practices

Workshop Options

  1. Basic Workshop
    Focus: EM operation and negative stain EM using SerialEM and the Talos L120C
  2. Advanced Workshop
    Focus: Single particle analysis (SPA) data acquisition using SerialEM and the Glacios

Workshops may be cancelled if fewer than three users register. Content, instructors, and scheduling are periodically adjusted based on participant feedback.

Upcoming Virtual Workshop

Date: Wednesday, July 30, 2025
Time: 10:00 AM – 4:00 PM
Topic: EM operation and Negative Stain EM using SerialEM and Talos L120C
Instructor: Jianfeng Lin

To register, please email Jianfeng Lin. Registered participants will receive training materials and a Zoom link in advance.
 

In-Person Training

YCR provides four types of in-person training tailored to varying expertise levels. Please refer to the YCR Policies page for details.

Training Types

  1. Negative Stain EM Training (Five 2-hour training sessions)
    Instruments: Talos L120C or Tecnai T12
  2. CryoEM Basic Training (Three 7-hour training sessions)
    Instrument: Glacios
  3. Advanced Training
    Example: SPA data acquisition on Glacios using SerialEM
  4. Refresher Training (<1 hour)
    For users returning to CryoEM after a break or needing updates

Registration for In-Person Training

Interested Yale users must first set up a Yale PPMS account by following the instructions, then request training by emailing one of the following staff members based on your preferred location:

Please include a completed EM Training Request Form in your email. External users should contact Jianfeng Lin directly.

Training Costs

Usage charges apply for in-person training according to the standard YCR fee schedule. For example:

  • CryoEM Basic Training on Glacios includes:
    • 3 hours of sample preparation training
    • 18 hours of EM/SerialEM training
    • Cost: $1,509 per internal trainee (group of two); $3,018 per internal trainee (individual)

Please refer to the training agenda for more information on content and scheduling.