The Yale Cryo-EM resource (Titan Krios), the Center for Cellular and Molecular Imaging (CCMI) at Yale School of Medicine (Tecnai T12 and Glacios, has a training program for beginners), and the Science Hill EM lab (Talos L120C) are integrative components of the Cryo-EM program at Yale University. A range of instruments is available to users to support research at different stages of their projects. Preliminary evaluation is performed utilizing the T12 using negative staining EM. Cryo-EM sample optimization and data collection may be performed at Glacios equipped with a K2 direct detector. The Talos L120C microscope can be used for both negative staining EM and Cryo-EM sample optimization. The Titan Krios should be primarily used for large-scale data collection on optimized samples.
The typical workflow for single particle projects.
Yale credentials are required to open the documents marked with *
- Practical guidelines for Yale Titan Krios
- Access policy and rate (FY2019)*
- Access request (long form for new projects)*
- An example of the long form*
- Access request (short form for existing projects)*
- Biohazard Questionnaire*
- Biosafety compliance
The price of the consumables is subject to change. Currently, it is $18 per grid.