The West campus Cryo-EM core (Titan Krios), the Center for Cellular and Molecular Imaging (CCMI) at Yale School of Medicine (Tecnai T12 and Glacios), and the Science Hill/YSB EM lab (Talos L120C and Glacios) are integrative components of the Cryo-EM program at Yale University. A range of instruments is available to users to support research at different stages of their projects. Preliminary evaluation is performed utilizing the T12 using negative staining EM. Cryo-EM sample optimization and data collection may be performed at Glacios equipped with a K3 direct detector. The Talos L120C microscope can be used for both negative staining EM and Cryo-EM sample optimization. The Titan Krios should be primarily used for large-scale data collection on optimized samples.
Training programs for beginners are provided at CCMI-YSM and ScienceHill EM lab.
Yale credentials are required to open the documents marked with *
- Get started as a Yale internal user*
- Set up Yale FOM account (all users need to complete this before requesting access).
- Access policy and rate*
- EM training request form* (an example of the form)
- Talos L120C or T12 access request form* (an example of the form)
- Glacios access request form* (an example of the form)
- Titan Krios access request form* (an example of the form)
- Practical guidelines for Yale Titan Krios
- Biohazard Questionnaire*
- Biosafety compliance
The price of the consumables is subject to change. Currently, it is $15.3 per set of autogrid ring and clip.
Computational needs are supported by Center for Research Computing. Please follow their guidelines and instructions.
You can subscribe to the Cryoem-journalclub mailing list to receive updates and announcements.
1. Go to this link:
https://mailman.yale.edu/mailman/listinfo/cryoem-journalclub
2. Fill in the top box on the page, your email address. Nothing else required.
3. Click Subscribe.
In addition, you can subscribe to the YSM_Structural_Biology_Seminar_Series mailing list by contacting titus.boggon@yale.edu
https://mailman.yale.edu/pipermail/ysm_structural_biology_seminar_serie…