User guidelines

The West campus Cryo-EM core (Titan Krios), the Center for Cellular and Molecular Imaging (CCMI) at Yale School of Medicine (Tecnai T12 and Glacios), and the Science Hill/YSB EM lab (Talos L120C and Glacios) are integrative components of the Cryo-EM program at Yale University. A range of instruments is available to users to support research at different stages of their projects. Preliminary evaluation is performed utilizing the T12 using negative staining EM. Cryo-EM sample optimization and data collection may be performed at Glacios equipped with a K3 direct detector. The Talos L120C microscope can be used for both negative staining EM and Cryo-EM sample optimization. The Titan Krios should be primarily used for large-scale data collection on optimized samples.

Training programs for beginners are provided at CCMI-YSM and ScienceHill EM lab.

You can subscribe to the Cryoem-journalclub mailing list to receive updates and announcements. 

1. Go to this link:
2. Fill in the top box on the page, your email address. Nothing else required.
3. Click Subscribe

The typical workflow for single particle projects.


Yale credentials are required to open the documents marked with *

The price of the consumables is subject to change. Currently, it is $20.35 per set of autogrid ring and clip.

Computational needs are supported by Center for Research Computing. Please follow their guidelines and instructions.

Prospective external users may find the general process as follows: