Yale TEM and support equipment

Yale CryoEM Resource-West campus Cryo-EM Core

FEI Titan Krios G2 300kV Transmission Electron Microscope

Specification highlights:

  • High-brightness X-FEG gun
  • ConstantPower lenses
  • Three condenser lens system for parallel sample illumination
  • Robotic Autoloader handles and stores up to 12 EM grids
  • Volta phase plate
  • GIF Quantum LS Imaging Filter Model 1967
  • Pre-GIF CETA2 indirect detection CMOS camera
  • Pre-GIF Falcon 3EC direct detection camera
  • Post-GIF K3 summit direct detection camera
  • EPU: Automated Data Acquisition Software for Single Particle Analysis
  • TEM Tomography Data Acquisition Software
  • Gatan Microscopy Suite® (GMS) 3.43

Third-party software:

  • SerialEM, for K3 only

View the Installation Timeline

Instruments for sample preparation

  • PELCO easiGlow™ Glow Discharge Cleaning System
    Optimized for cleaning TEM grids
  • FEI Vitrobot™ Mark IV

Yale CryoEM Resource-YSM Center for Cellular and Molecular Imaging

FEI T12, Thermo Scientific Glacios, SPT Labtech Chameleon vitrification robot 

Specification highlights of 200kV CCMI-SHM-Glacios:

  • High-brightness X-FEG gun
  • ConstantPower Objective lens
  • Robotic Autoloader handles and stores up to 12 EM grids
  • Cryo-stage with single axis holder
  • Volta phase plate
  • CETA CMOS camera
  • K3 summit direct detection camera
  • EPU Software (for CETA)
  • SerialEM (for K3)

Specification highlights of 120kV CCMI-SHM-T12:

  • LaB6 cathode
  • Cryo-stage with side entry holder
  • Gatan CCD camera
  • SerialEM

SPT Labtech Chameleon:

Yale CryoEM Resource-ScienceHill EM lab

Thermo Scientific Talos L120C, Thermo Scientific Glacios

Specification highlights of 200kV ScienceHill-YSB-Glacios:

  • High-brightness X-FEG gun
  • ConstantPower Objective lens
  • Robotic Autoloader handles and stores up to 12 EM grids
  • Cryo-stage with single axis holder
  • CETA-D CMOS camera
  • K3 summit direct detection camera
  • EPU-D Software (for MircoED)
  • SerialEM (for K3)

Specification highlights of 120kV ScienceHill-Bass-Talos L120c:

  • LaB6 cathode
  • ConstantPower Objective lens
  • Cryo-stage with side entry holder
  • Motorized Apertures
  • Digital FluCam
  • CETA CMOS camera
  • SerialEM

Other TEM and support equipment
Here is an incomplete list of EM related instruments at other Yale facilities or labs.
 

  • Talos L120c at Department of Neuroscience (co-managed by CCMI)

  • T12, TF20, Zeiss dual beam, and Aquilos Cryo-FIB at CCMI

Tecnai T12 BioTwin (kept at 80kV), Tecnai F20 X-Twin, Zeiss Crossbeam 550 FIB-SEM, Aquilos 2

  • JEOL TEM at West campus

JEOL JEM 1400-plus 120 kV (kept at 80kV); LaB6 filament; bottom-mount AMT XR611 camera. Located at ISTC 006F.  Potential users should contact Chenxiang Lin (chenxiang.lin@yale.edu).

  • CryoFIB at West campus

Thermo Scientific Aquilos at Jun Liu lab

Addtional equipment includes Leica GP2 Plunge Freezer and  Leica Cryo-CLEM.

  • Yale Institute for Nanoscience & Quantum Engineering

FEI Tecnai Osiris 200kV TEM

  • West Campus Materials Characterization Core

Thermo Scientific Helios FIB SEM Dual Beam