Yale TEM and support equipment

300-kV Titan Krios G2 Transmission Electron Microscope 

• X-FEG gun
• ConstantPower lenses
• Three condenser lens system for parallel sample illumination
• Autoloader handles and stores up to 12 EM grids
• Volta phase plate
• GIF Quantum LS Imaging Filter Model 1967
• Pre-GIF CETA2 indirect detection CMOS camera
• Pre-GIF Falcon 3EC direct detection camera
• Post-GIF K3 summit direct detection camera
• EPU: Automated Data Acquisition Software for Single Particle Analysis
• TEM Tomography Data Acquisition Software
• Gatan Microscopy Suite® (GMS) 3.43
• SerialEM (for K3)

Instruments for sample preparation

              o PELCO easiGlow™ Glow Discharge Cleaning System
              o FEI Vitrobot™ Mark IV

200-kV YSM-Glacios:

• X-FEG gun
• ConstantPower Objective lens
• Autoloader handles and stores up to 12 EM grids
• Cryo-stage with single axis holder
• Volta phase plate
• CETA CMOS camera
• K3 direct detection camera
• EPU Software (for CETA)
• SerialEM (for K3)

120-kV YSM-Tecnai T12:

• LaB6 cathode
• Cryo-stage with side entry holder
• Gatan CCD camera
• SerialEM

Instruments for sample preparation

             o SPT Labtech Chameleon

             o FEI Vitrobot™ Mark IV

             o Leica EM ACE600 High-Vacuum Coater

             o Gatan Solarus Advanced Plasma System (Model 950)

200-kV SH-Glacios:

• X-FEG gun
• ConstantPower Objective lens
• Autoloader handles and stores up to 12 EM grids
• Cryo-stage with single axis holder
• CETA-D CMOS camera
• K3 summit direct detection camera
• EPU-D Software (for MircoED)
• SerialEM (for K3)

120-kV SH-Talos L120C:

• LaB6 cathode
• ConstantPower Objective lens
• Cryo-stage with side entry holder
• Motorized Apertures
• Digital FluCam
• CETA CMOS camera
• SerialEM

Instruments for sample preparation

              o PELCO easiGlow™ Glow Discharge Cleaning System

              o FEI Vitrobot™ Mark IV

              o Fischione: Model 1070 NanoClean

              o UVOCS: UV Ozone Cleaning System (model T10X10/OES)

              o Cressington: Carbon Coater 208 

Here is an incomplete list of EM related instruments at other Yale facilities or labs.

• Aquilos CryoFIB, Leica GP2 Plunge Freezer, and  Leica Cryo-CLEM at Jun Liu lab

• Aquilos CryoFIB, Zeiss dual beam, T12, and TF20 at CCMI

• Talos L120c at Department of Neuroscience (co-managed by CCMI)

• Zeiss eFIB-SEM at FIB-SEM Collaboration core

• JEOL JEM 1400-plus at Chenxiang Lin Lab

• FEI Tecnai Osiris 200kV TEM YINQE

• Thermo Scientific Spectra Ultra STEM and Helios G4 UX DualBeam System at ACEM core