About CryoEM

Cryo-electron microscopy (cryoEM) is a powerful technique for studying the structures of biological samples that are rapidly frozen (vitrified) at liquid nitrogen temperatures. It encompasses a range of applications, including single-particle analysis (SPA), cryo-electron tomography (cryoET), 2D electron crystallography, and microcrystal electron diffraction (MicroED).

1. Single particle analysis of purified protein complexes

Cheng Y, Grigorieff N, Penczek PA & Walz, T. A primer to single-particle cryo-electron microscopy. Cell. 2015, 161: 438–449.

2. Cryo electron tomography

Lučič V, Rigort A, Baumeister W. Cryo-electron tomography: the challenge of doing structural biology in situ. J Cell Biol. 2013, 202: 407–419.

3. MicroED

Brent L. Nannenga & Tamir Gonen The cryo-EM method microcrystal electron diffraction (MicroED). Nature Methods. 2019, 16: 369–379.