Scientists use Cryo-EM techniques to study structures of vitrified biological samples frozen at liquid nitrogen temperature. There are a variety of applications, including single particle analysis, electron cryo-tomography, 2D electron crystallography and MicroED (3D electron crystallography of nanoscale crystals). The Yale Titan Krios is primarily used for the former two applications. The Glacios at Science Hill is equipped with MicroED package.
1. Single particle analysis of purified protein complexes
Cheng, Y., Grigorieff, N., Penczek, P. A. & Walz, T. A primer to single-particle cryo-electron microscopy. Cell 161, 438–449 (2015).
2. Electron cryo-tomography
Lučič V, Rigort A, Baumeister W (2013) Cryo-electron tomography: the challenge of doing structural biology in situ. J Cell Biol 202(3):407–419.
Brent L. Nannenga & Tamir Gonen The cryo-EM method microcrystal electron diffraction (MicroED). Nature Methods volume 16, pages369–379 (2019)