Known glitches

1. EF-CCD does not show up in the camera panel of Titan UI.

what to do: close the programs in reverse order of start-up and try again, i.e. close TIA –> open TIA. If it doesn’t work, close TIA –> close GMS. Select Exist without saving when asked about GIF alignment upon closing GMS. If this message window does not show up, check Task manager, find DigitalMicrograph process and force-end it.Select Exist without saving when asked about GIF alignment upon closing GMS.

2. K2 fails to insert after annealing.

what to do: report to staff. Authorized users may perform the fix involving hardware reboot.

3. K2 loses half of the signal (one side of the image is dark).

what to do: report to staff. Authorized users may perform the fix involving hardware reboot.

4. TEM server crashes as “BrickBox application process ‘Tem’ ended unexpectedly”. Such crashes usually cause High Tension to turn off and Autoloader to become uninitialized.

what to do: report to staff. Authorized users may perform the fix involving restarting the Tem Server.

5. A fixed vertical line shows up near the right edge of the K2 camera view.

what to do: stop data collection –> In DM, click the gear icon in the camera monitor (lower left). If you don’t see the gear icon, go to menu bar/help/user mode, select Power User –> In the popup menu, click System Off, wait 15s, the camera status indicator should turn grey –> click System On, wait around 15min, messages about uploading reference images will show up during the process, click upload –> after the system finishes startup, the indicator should turn green again –> check camera temperature, wait until it reaches -20C before resuming operation. Capture a test image, the line should go away. Otherwise,  cease operation and report to the staff .

6. GIF tuning exit too early

what to do: Restart DM (refer to operational guide for the correct sequence).

7. The green button does not light up when the nanocab is inserted. In the user interface, autoloader vacuum is off and autoloader becomes uninitialized.

what to do: click Vacuum On in the autoloader flap-out menu, then click Initialize when the button is clickable. Resume operation if it returns to normal. Otherwise, cease operation and report to the staff or Thermo customer service.

8. SerialEM crashes unexpectedly.

what to do: check DM information area (near bottom), to see if there is any error message –> capture a test image in DM, confirm DM is operating normally. Restart DM if necessary (run Tune GIF or Center ZLP after DM restarts ) –> restart SerialEM –> load your customized setting file, read and open the navigator file –> check Low dose mode, double check all the camera setup parameters, modify the image saving number if necessary –> resume acquisition.