User guidelines

The Yale Cryo-EM resource (Titan Krios), the Center for Cellular and Molecular Imaging (CCMI) at Yale School of Medicine (Tecnai T12 and TF20, has a training program for beginners), and the Science Hill EM lab (Talos L120C) are integrative components of the Cryo-EM program at Yale University. A range of instruments is available to users to support research at different stages of their projects. Preliminary evaluation is performed utilizing the T12 using negative staining EM. Cryo-EM sample optimization and small scale data collection may be performed at TF20 equipped with a K2 direct detector. The new Talos L120C microscope can be used for both negative staining EM and Cryo-EM sample optimization. The Titan Krios should be primarily used for large-scale data collection on optimized samples.

Yale credentials are required to open some of the documents.

Practical guidelines
https://yale.box.com/s/9jr6gq0ssue55gq2962q60x2r0f038c0
Access policy and rates
https://yale.box.com/s/98sezfuvys3dgat9glxjdvl4apirxjiz
Access request (long form for new projects)
https://yale.box.com/s/ih5793matdwhvje2v0kzrxbnax73csh0
An example of the long form
https://yale.box.com/s/jwg9mcauho8yleqoquqxfsdb9yq6jd9x
Access request (short form for existing projects)
https://yale.box.com/s/g5cl7u91fu5bywo7rx24jjj8g03xkun6

• The price of the consumables is subject to change. Currently, it is $17 per grid.


Computational needs are supported by Center for Research Computing. Please follow their guidelines and instructions.