1. Parameters related to the optical system
Acceleration voltage: 300kV
Cs of Cryo-TWIN objective lens: 2.7mm
Available C2 aperture sizes (μm): 150, 100, 70, 50
Available objective aperture sizes (μm): 100, 70
2. Parameters related to pre-GIF Falcon 3EC camera
Dimension: 4096 x 4096 pixels
Physical pixel side length: 14μm
Mag table (pixel sizes calibrated using reconstructions of T20S):
- 47000x 1.706A
- 59000x 1.36A
- 75000x 1.07A
- 96000x 0.831A
Magnification distortion (estimated in June 2017 using method mag_distortion_estimate)
• SA 75000x
The following distortion parameters were found :-
Distortion Angle = 88.6
Major Scale = 1.002
Minor Scale = 0.998
Stretch only parameters would be as follows :-
Distortion Angle = 88.6
Major Scale = 1.004
Minor Scale = 1.000
Corrected Pixel Size = 1.068
The Total Distortion = .35%
3. Parameters related to post-GIF K2 summit camera
Dimension: 3838 x 3710 physical pixels; 7676 x 7420 super-resolution pixels
Physical pixel side length: 5μm
Mag table (physical pixel sizes, calibrated using reconstructions of T20S):
81000x 1.715A
105kx 1.333A
130kx 1.05A
165kx 0.822A
Magnification distortion (estimated in June 2017 using method mag_distortion_estimate, super-resolution pixel sizes are used below):
• 165kx
The following distortion parameters were found :-
Distortion Angle = 147.3
Major Scale = 1.005
Minor Scale = 0.995
Stretch only parameters would be as follows :-
Distortion Angle = 147.3
Major Scale = 1.010
Minor Scale = 1.000
Corrected Pixel Size = 0.409
The Total Distortion = 1.00%
• 130kx
The following distortion parameters were found :-
Distortion Angle = 141.1
Major Scale = 1.005
Minor Scale = 0.996
Stretch only parameters would be as follows :-
Distortion Angle = 141.1
Major Scale = 1.009
Minor Scale = 1.000
Corrected Pixel Size = 0.523
The Total Distortion = .90%
• 105kx
The following distortion parameters were found :-
Distortion Angle = 143.0
Major Scale = 1.007
Minor Scale = 0.994
Stretch only parameters would be as follows :-
Distortion Angle = 143.0
Major Scale = 1.013
Minor Scale = 1.000
Corrected Pixel Size = 0.662
The Total Distortion = 1.30%
• 81kx
The following distortion parameters were found :-
Distortion Angle = 149.5
Major Scale = 1.004
Minor Scale = 0.996
Stretch only parameters would be as follows :-
Distortion Angle = 149.5
Major Scale = 1.008
Minor Scale = 1.000
Corrected Pixel Size = 0.854
The Total Distortion = .75%
MTF star file (estimated in June 2017 using method FindDQE)