Useful parameters

1. Parameters related to the optical system

Acceleration voltage: 300kV

Cs of Cryo-TWIN objective lens: 2.7mm

Available C2 aperture sizes (μm): 150, 100, 70, 50

Available objective aperture sizes (μm): 100, 70

2. Parameters related to pre-GIF Falcon 3EC camera

Dimension: 4096 x 4096 pixels

Physical pixel side length: 14μm

Mag table (pixel sizes calibrated using reconstructions of T20S):
47000x  1.706A
59000x  1.36A
75000x  1.07A
96000x  0.831A

Magnification distortion (estimated in June 2017 using method mag_distortion_estimate)

• SA 75000x

The following distortion parameters were found :-
Distortion Angle     = 88.6
Major Scale          = 1.002
Minor Scale          = 0.998
Stretch only parameters would be as follows :-
Distortion Angle     = 88.6
Major Scale          = 1.004
Minor Scale          = 1.000
Corrected Pixel Size = 1.068
The Total Distortion = .35%

3. Parameters related to post-GIF K2 summit camera

Dimension: 3838 x 3710 physical pixels; 7676 x 7420 super-resolution pixels

Physical pixel side length: 5μm

Mag table (physical pixel sizes, calibrated using reconstructions of T20S):
81000x  1.715A
105kx  1.333A
130kx  1.05A
165kx  0.822A

Magnification distortion (estimated in June 2017 using method mag_distortion_estimate, super-resolution pixel sizes are used below):

• 165kx

The following distortion parameters were found :-
Distortion Angle     = 147.3
Major Scale          = 1.005
Minor Scale          = 0.995
Stretch only parameters would be as follows :-
Distortion Angle     = 147.3
Major Scale          = 1.010
Minor Scale          = 1.000
Corrected Pixel Size = 0.409
The Total Distortion = 1.00%

• 130kx

The following distortion parameters were found :-
Distortion Angle    = 141.1
Major Scale          = 1.005
Minor Scale          = 0.996
Stretch only parameters would be as follows :-
Distortion Angle     = 141.1
Major Scale          = 1.009
Minor Scale          = 1.000
Corrected Pixel Size = 0.523
The Total Distortion = .90%

• 105kx

The following distortion parameters were found :-
Distortion Angle     = 143.0
Major Scale          = 1.007
Minor Scale          = 0.994
Stretch only parameters would be as follows :-
Distortion Angle     = 143.0
Major Scale          = 1.013
Minor Scale          = 1.000
Corrected Pixel Size = 0.662
The Total Distortion = 1.30%

• 81kx

The following distortion parameters were found :-
Distortion Angle     = 149.5
Major Scale          = 1.004
Minor Scale          = 0.996
Stretch only parameters would be as follows :-
Distortion Angle     = 149.5
Major Scale          = 1.008
Minor Scale          = 1.000
Corrected Pixel Size = 0.854
The Total Distortion = .75%

MTF star file (estimated in June 2017 using method FindDQE)